Glucose and Gluconate Metabolism in an Escherichia coli Mutant Lacking Phosphoglucose Isomerase
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چکیده
منابع مشابه
Glucose and gluconate metabolism in a mutant of Escherichia coli lacking gluconate-6-phosphate dehydrase.
A mutant lacking gluconate-6-phosphate dehydrase (the first enzyme of the Entner-Doudoroff pathway) was isolated after ethyl methane sulfonate mutagenesis of Escherichia coli. Other enzymes of gluconate metabolism (gluconokinase, gluconate-6-phosphate dehydrogenase, and 2-keto-3-deoxygluconate-6-phosphate aldolase) were present in the mutant. When the mutant was grown on gluconate-1-(14)C, alan...
متن کاملThe accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase.
When presented with glucose, an Escherichia coli mutant lacking both phosphoglucose isomerase and glucose 6-phosphate dehydrogenase accumulated, internally, high concentrations (approximately 50 mM) of glucose 6-phosphate. It also accumulated glucose in polysaccharide form. Other metabolism of glucose, if any, was very restricted. The accumulation of glucose 6-phosphate was associated with inhi...
متن کاملNew phosphoglucose isomerase mutants of Escherichia coli.
The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those descr...
متن کاملResponses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts.
The responses of Escherichia coli central carbon metabolism to knockout mutations in phosphoglucose isomerase and glucose-6-phosphate (G6P) dehydrogenase genes were investigated by using glucose- and ammonia-limited chemostats. The metabolic network structures and intracellular carbon fluxes in the wild type and in the knockout mutants were characterized by using the complementary methods of fl...
متن کاملReduced enzyme activity and starch level in an induced mutant of chloroplast phosphoglucose isomerase.
Ethyl methane sulfonate treatment was used to induce a mutation in the nuclear gene encoding the chloroplast isozyme of phosphoglucose isomerase in Clarkia xantiana. The mutation, which proved allelic to wild type activity, was backcrossed to wild type for five generations so that the two could be compared in a near isogenic background. An immunological analysis showed that the mutant, when hom...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1967
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.93.5.1571-1578.1967